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KMID : 0942820050040010007
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Journal of Korean Brain Tumor Society
2005 Volume.4 No. 1 p.7 ~ p.12
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Immunohistochemical and Molecular Biological Analysis of the Recurrent Vestibular Schwannoma
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Lee Jae-Won
Kim Mi-Hyun
Ahn Sung-Ki
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Abstract
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Objective: In generally, diagnosis of schwannoma is achieved by observation for tumor location, symptoms, radiological studies or surgical findings. However, it is reported that schwannoma can present a wide spectrum of histological patterns and show uncommon variants. The hypothesis of this study is that the tumor of a patient showing a second attack may show unexpected specific characteristics or phenotypic expression for genes associated with merlin or schwannomin. According to this hypothesis, the purpose of this study is to observe the progression state of vestibular schwannoma with molecular biological analysis.
Methods: Histological and molecular biological analysis using immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR) method were carried out using collected tumor tissues from a patient with recurrent vestibular schwannoma.
Results: Collected tumor tissues showed a histologically typical schwannoma with spindle-shaped cells. Immunohistochemical study revealed that the tumor tissues were stained with S-100, vimentin, CD44, CD34 and desmin antibodies. However, glial fibrillary acidic protein(GFAP), HMB45 and cytokeratin antibodies were not reacted. In contrast, expression of GFAP was only examined in normal nerve tissue. Schwannoma related gene expression profiling was also examined. Neurofibromatosis type 2(NF2) and GFAP mRNA expressions in vestibular schwannoma were relatively lower than normal nerve tissues. Furthermore, the vestibular schwannoma expressed high level of FIR mRNA, which regulates neurite remodeling, compared to normal nerve tissues.
Conclusion: These results provide evidence that benign vestibular schwannoma tissue may progress to malignant tumor and suggest that these diagnostic options for managing a vestibular schwannoma might be supported to make an appropriate diagnosis and best treatment.
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KEYWORD
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Vestibular schwannoma, Merlin, Schwannomin, Neurofibromatosis type 2, immunohistochemistry
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